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rabbit polyclonal antibody against ror2 (Abnova)

Name: rabbit polyclonal antibody against ror2
Brand: Abnova
Rating: 90 (1 reviews)

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Abnova rabbit polyclonal antibody against ror2

(A) Immunohistochemical analysis of human specimens of urothelial carcinoma of the bladder for the expression of Wnt5a, <t>ROR2,</t> CTHRC1 and E-cadherin. Left column, tissue sections from a representative case of low grade urothelial carcinoma; the right column, tissue sections from a representative case of high grade urothelial carcinoma. The middle column represents the trend of expression for each protein in all 15 samples. The expression of Wnt5a, <t>ROR2,</t> and CTHRC1 increases in high grade tumors while E-cadherin shows an opposite trend. Bar=50 μm. (B) Statistical analysis was performed to investigate the correlation between tumor histological grade and immunostaining for Wnt5a, Ror2, CTHRC1, and E-cadherin. Statistical significance was tested at an alpha of 0.05. The software PASW Statistics 18 was used for data analysis (Pearson Education, New York City, NY).

Rabbit Polyclonal Antibody Against Ror2, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibody against ror2/product/Abnova
Average 90 stars, based on 1 article reviews

rabbit polyclonal antibody against ror2 - by Bioz Stars, 2026-03

90/100 stars

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1) Product Images from "Wnt5a / planar cell polarity signaling pathway in urothelial carcinoma, a potential prognostic biomarker"

Article Title: Wnt5a / planar cell polarity signaling pathway in urothelial carcinoma, a potential prognostic biomarker

Journal: Oncotarget

doi: 10.18632/oncotarget.15877

(A) Immunohistochemical analysis of human specimens of urothelial carcinoma of the bladder for the expression of Wnt5a, ROR2, CTHRC1 and E-cadherin. Left column, tissue sections from a representative case of low grade urothelial carcinoma; the right column, tissue sections from a representative case of high grade urothelial carcinoma. The middle column represents the trend of expression for each protein in all 15 samples. The expression of Wnt5a, ROR2, and CTHRC1 increases in high grade tumors while E-cadherin shows an opposite trend. Bar=50 μm. (B) Statistical analysis was performed to investigate the correlation between tumor histological grade and immunostaining for Wnt5a, Ror2, CTHRC1, and E-cadherin. Statistical significance was tested at an alpha of 0.05. The software PASW Statistics 18 was used for data analysis (Pearson Education, New York City, NY).

Figure Legend Snippet: (A) Immunohistochemical analysis of human specimens of urothelial carcinoma of the bladder for the expression of Wnt5a, ROR2, CTHRC1 and E-cadherin. Left column, tissue sections from a representative case of low grade urothelial carcinoma; the right column, tissue sections from a representative case of high grade urothelial carcinoma. The middle column represents the trend of expression for each protein in all 15 samples. The expression of Wnt5a, ROR2, and CTHRC1 increases in high grade tumors while E-cadherin shows an opposite trend. Bar=50 μm. (B) Statistical analysis was performed to investigate the correlation between tumor histological grade and immunostaining for Wnt5a, Ror2, CTHRC1, and E-cadherin. Statistical significance was tested at an alpha of 0.05. The software PASW Statistics 18 was used for data analysis (Pearson Education, New York City, NY).

Techniques Used: Immunohistochemical staining, Expressing, Immunostaining, Software

Figure Legend Snippet: Wnt5a (A) , Ror2 and CTHRC1 (B) , E-cadherin and vimentin (C) , in RT4, J82 and T24 urothelial carcinoma cell lines.

Techniques Used:

Top row: H&E stain shows morphological differences between each cell line. Middle row: confocal microscopy images show the expression of Wnt5a (green) for each cell line. Bottom row: confocal microscopy image of the merge expression of Ror2 (green) and CTHRC1 (red) for each cell line. Although the co-expression and colocalization of Ror2 and CTHRC1 is present in all cell lines, it is clearest for RT4 and J82 cell lines.

Figure Legend Snippet: Top row: H&E stain shows morphological differences between each cell line. Middle row: confocal microscopy images show the expression of Wnt5a (green) for each cell line. Bottom row: confocal microscopy image of the merge expression of Ror2 (green) and CTHRC1 (red) for each cell line. Although the co-expression and colocalization of Ror2 and CTHRC1 is present in all cell lines, it is clearest for RT4 and J82 cell lines.

Techniques Used: Staining, Confocal Microscopy, Expressing

Figure Legend Snippet: Primer sequences used for real-time RT-PCR analyses

Techniques Used: Quantitative RT-PCR

Image Search Results

Cross sections were immunostained with anti-Wnt5a (A–C), anti-Fzd2 (D–F), or anti-Ror2 (G–I). A, D, G: Stage 57. Cells positive for Ror2 (G; arrows) are scattered only in the larval epithelium (LE). They possess the brush border (bb) on the apical surface (Inset). B, E, H: Stage 61. Cells positive for Wnt5a (B) and Fzd2 (E) are broadly distributed in every tissue except for the degenerating larval epithelium, whereas a strong immunoreactivity for Ror2 is localized in islets of the adult epithelium (AE) (H). C, F, I: Stage 66. Immunoreactivity for each protein becomes weaker. CT: connective tissue; M: muscles; Scale bars: 20 µm.

Journal: PLoS ONE

Article Title: Thyroid Hormone-Regulated Wnt5a/Ror2 Signaling Is Essential for Dedifferentiation of Larval Epithelial Cells into Adult Stem Cells in the Xenopus laevis Intestine

doi: 10.1371/journal.pone.0107611

Figure Lengend Snippet: Cross sections were immunostained with anti-Wnt5a (A–C), anti-Fzd2 (D–F), or anti-Ror2 (G–I). A, D, G: Stage 57. Cells positive for Ror2 (G; arrows) are scattered only in the larval epithelium (LE). They possess the brush border (bb) on the apical surface (Inset). B, E, H: Stage 61. Cells positive for Wnt5a (B) and Fzd2 (E) are broadly distributed in every tissue except for the degenerating larval epithelium, whereas a strong immunoreactivity for Ror2 is localized in islets of the adult epithelium (AE) (H). C, F, I: Stage 66. Immunoreactivity for each protein becomes weaker. CT: connective tissue; M: muscles; Scale bars: 20 µm.

Article Snippet: The elutes were subjected to Western blotting using a rabbit polyclonal antibody against human Ror2 (diluted 1∶250; Sigma-Aldrich).

Techniques:

Cross sections were double-immunostained with anti-Ror2 (green) and anti-Msi1 (A; red), anti-CK19 (B; red), or anti-PCNA (C; red) antibodies, and counterstained with DAPI. Adult epithelial cells (AE) coexpress Ror2 and Msi1, CK19, or PCNA (arrows). CT: connective tissue; LE: larval epithelium. Scale bars: 20 µm.

Journal: PLoS ONE

Article Title: Thyroid Hormone-Regulated Wnt5a/Ror2 Signaling Is Essential for Dedifferentiation of Larval Epithelial Cells into Adult Stem Cells in the Xenopus laevis Intestine

doi: 10.1371/journal.pone.0107611

Figure Lengend Snippet: Cross sections were double-immunostained with anti-Ror2 (green) and anti-Msi1 (A; red), anti-CK19 (B; red), or anti-PCNA (C; red) antibodies, and counterstained with DAPI. Adult epithelial cells (AE) coexpress Ror2 and Msi1, CK19, or PCNA (arrows). CT: connective tissue; LE: larval epithelium. Scale bars: 20 µm.

Article Snippet: The elutes were subjected to Western blotting using a rabbit polyclonal antibody against human Ror2 (diluted 1∶250; Sigma-Aldrich).

Techniques:

Cross sections were immunostained with anti-Ror2 (brown) or stained with MG-PY (blue and red). A–E: Stage 60. Epithelial cells positive for Ror2 show various morphologies and are scattered in the larval epithelium (LE) (A; arrows). They include simple columnar cells possessing the brush border (bb) (B), triangular-shaped cells (C), and roundish islet-like cells close to the connective tissue (CT) (D, E), all of which are strongly stained red with MG-PY. F: Stage 61. Ror2 expression is localized in islets of the adult epithelium (AE) stained red with MG-PY. G: Stage 62. Numerous adult epithelial cells are positive for Ror2, although the immunoreactivity per each cell gradually becomes weaker. Scale bars: 20 µm.

Journal: PLoS ONE

Article Title: Thyroid Hormone-Regulated Wnt5a/Ror2 Signaling Is Essential for Dedifferentiation of Larval Epithelial Cells into Adult Stem Cells in the Xenopus laevis Intestine

doi: 10.1371/journal.pone.0107611

Figure Lengend Snippet: Cross sections were immunostained with anti-Ror2 (brown) or stained with MG-PY (blue and red). A–E: Stage 60. Epithelial cells positive for Ror2 show various morphologies and are scattered in the larval epithelium (LE) (A; arrows). They include simple columnar cells possessing the brush border (bb) (B), triangular-shaped cells (C), and roundish islet-like cells close to the connective tissue (CT) (D, E), all of which are strongly stained red with MG-PY. F: Stage 61. Ror2 expression is localized in islets of the adult epithelium (AE) stained red with MG-PY. G: Stage 62. Numerous adult epithelial cells are positive for Ror2, although the immunoreactivity per each cell gradually becomes weaker. Scale bars: 20 µm.

Article Snippet: The elutes were subjected to Western blotting using a rabbit polyclonal antibody against human Ror2 (diluted 1∶250; Sigma-Aldrich).

Techniques: Staining, Expressing

Cross sections were stained with MG-PY (A, C) or immunostained with anti-Ror2 (B, D). A, B: Control intestines. Cells positive for Ror2 are scattered in the simple columnar epithelium (B; arrow). C, D: Intestines cultured with the addition of Wnt5a. Epithelial cells positive for Ror2 change in morphology (arrows) and often invaginate into the connective tissue (CT). Scale bars: 20 µm.

Journal: PLoS ONE

Article Title: Thyroid Hormone-Regulated Wnt5a/Ror2 Signaling Is Essential for Dedifferentiation of Larval Epithelial Cells into Adult Stem Cells in the Xenopus laevis Intestine

doi: 10.1371/journal.pone.0107611

Figure Lengend Snippet: Cross sections were stained with MG-PY (A, C) or immunostained with anti-Ror2 (B, D). A, B: Control intestines. Cells positive for Ror2 are scattered in the simple columnar epithelium (B; arrow). C, D: Intestines cultured with the addition of Wnt5a. Epithelial cells positive for Ror2 change in morphology (arrows) and often invaginate into the connective tissue (CT). Scale bars: 20 µm.

Article Snippet: The elutes were subjected to Western blotting using a rabbit polyclonal antibody against human Ror2 (diluted 1∶250; Sigma-Aldrich).

Techniques: Staining, Cell Culture

Cross sections were double-immunostained with anti-Ror2 (green) and anti-Msi1 (A, C; red) or anti-PCNA (B, D; red) antibodies, and counterstained with DAPI. A, B: Control intestines. The simple columnar epithelium (E) remains negative for Msi1 (A). Proliferating cells positive for PCNA are very few, if any (B). C, D: Intestines cultured with the addition of Wnt5a. Epithelial cells positive for Ror2 invaginate into the connective tissue (CT) but remain negative for Msi1 (C). Proliferating cells are more numerous than those in the control intestines and are also detectable in cells negative for Ror2 (D; arrows). Scale bars: 20 µm.

Journal: PLoS ONE

Article Title: Thyroid Hormone-Regulated Wnt5a/Ror2 Signaling Is Essential for Dedifferentiation of Larval Epithelial Cells into Adult Stem Cells in the Xenopus laevis Intestine

doi: 10.1371/journal.pone.0107611

Figure Lengend Snippet: Cross sections were double-immunostained with anti-Ror2 (green) and anti-Msi1 (A, C; red) or anti-PCNA (B, D; red) antibodies, and counterstained with DAPI. A, B: Control intestines. The simple columnar epithelium (E) remains negative for Msi1 (A). Proliferating cells positive for PCNA are very few, if any (B). C, D: Intestines cultured with the addition of Wnt5a. Epithelial cells positive for Ror2 invaginate into the connective tissue (CT) but remain negative for Msi1 (C). Proliferating cells are more numerous than those in the control intestines and are also detectable in cells negative for Ror2 (D; arrows). Scale bars: 20 µm.

Article Snippet: The elutes were subjected to Western blotting using a rabbit polyclonal antibody against human Ror2 (diluted 1∶250; Sigma-Aldrich).

Techniques: Cell Culture

Cross sections were stained with MG-PY (A, C) or immunostained with anti-Ror2 (B, D). A, B: Intestines treated with 20 nM T3. Adult stem/progenitor cells stained strongly red with MG-PY and positive for Ror2 (A, B; arrow) invaginate into the connective tissue (CT). C, D: T3-treated intestines cultured with the addition of anti-Wnt5a antibody. The epithelium (E) remains a single layer, where cells positive for Ror2 are scattered (D; arrows). Scale bars: 20 µm.

Journal: PLoS ONE

Article Title: Thyroid Hormone-Regulated Wnt5a/Ror2 Signaling Is Essential for Dedifferentiation of Larval Epithelial Cells into Adult Stem Cells in the Xenopus laevis Intestine

doi: 10.1371/journal.pone.0107611

Figure Lengend Snippet: Cross sections were stained with MG-PY (A, C) or immunostained with anti-Ror2 (B, D). A, B: Intestines treated with 20 nM T3. Adult stem/progenitor cells stained strongly red with MG-PY and positive for Ror2 (A, B; arrow) invaginate into the connective tissue (CT). C, D: T3-treated intestines cultured with the addition of anti-Wnt5a antibody. The epithelium (E) remains a single layer, where cells positive for Ror2 are scattered (D; arrows). Scale bars: 20 µm.

Article Snippet: The elutes were subjected to Western blotting using a rabbit polyclonal antibody against human Ror2 (diluted 1∶250; Sigma-Aldrich).

Techniques: Staining, Cell Culture

Journal: PLoS ONE

Article Title: Thyroid Hormone-Regulated Wnt5a/Ror2 Signaling Is Essential for Dedifferentiation of Larval Epithelial Cells into Adult Stem Cells in the Xenopus laevis Intestine

doi: 10.1371/journal.pone.0107611

Figure Lengend Snippet: Cross sections were double-immunostained with anti-Ror2 (green) and anti-Msi1 (A, C; red) or anti-PCNA (B, D; red) antibodies, and counterstained with DAPI. A, B: Intestines treated with 20 nM T3. Epithelial cells (E) invaginating into the connective tissue (CT) are double positive for Ror2 and Msi1 (A; arrow) and actively proliferate (B). C, D: T3-treated intestines cultured with the addition of anti-Wnt5a antibody. Epithelial cells positive for Ror2 remain negative for Msi1 (C). Proliferating cells are fewer than those in the T3-treated intestines in the absence of the antibody (D). Scale bars: 20 µm.

Article Snippet: The elutes were subjected to Western blotting using a rabbit polyclonal antibody against human Ror2 (diluted 1∶250; Sigma-Aldrich).

Techniques: Cell Culture

Larval absorptive cells expressing Ror2 are scattered in the simple columnar epithelium until stage 59. At stage 60, thyroid hormone up-regulates the expression of Wnt5a, whose protein binds to Ror2 of the absorptive cells and changes their morphology from simple columnar to roundish cells close to the connective tissue. This Wnt5a/Ror2 signaling is not sufficient but essential for epithelial dedifferentiation into the stem cells. In addition, Wnt5a promotes cell proliferation via receptors other than Ror2.

Journal: PLoS ONE

Article Title: Thyroid Hormone-Regulated Wnt5a/Ror2 Signaling Is Essential for Dedifferentiation of Larval Epithelial Cells into Adult Stem Cells in the Xenopus laevis Intestine

doi: 10.1371/journal.pone.0107611

Figure Lengend Snippet: Larval absorptive cells expressing Ror2 are scattered in the simple columnar epithelium until stage 59. At stage 60, thyroid hormone up-regulates the expression of Wnt5a, whose protein binds to Ror2 of the absorptive cells and changes their morphology from simple columnar to roundish cells close to the connective tissue. This Wnt5a/Ror2 signaling is not sufficient but essential for epithelial dedifferentiation into the stem cells. In addition, Wnt5a promotes cell proliferation via receptors other than Ror2.

Article Snippet: The elutes were subjected to Western blotting using a rabbit polyclonal antibody against human Ror2 (diluted 1∶250; Sigma-Aldrich).

Techniques: Expressing

Journal: Oncotarget

Article Title: Wnt5a / planar cell polarity signaling pathway in urothelial carcinoma, a potential prognostic biomarker

doi: 10.18632/oncotarget.15877

Figure Lengend Snippet: (A) Immunohistochemical analysis of human specimens of urothelial carcinoma of the bladder for the expression of Wnt5a, ROR2, CTHRC1 and E-cadherin. Left column, tissue sections from a representative case of low grade urothelial carcinoma; the right column, tissue sections from a representative case of high grade urothelial carcinoma. The middle column represents the trend of expression for each protein in all 15 samples. The expression of Wnt5a, ROR2, and CTHRC1 increases in high grade tumors while E-cadherin shows an opposite trend. Bar=50 μm. (B) Statistical analysis was performed to investigate the correlation between tumor histological grade and immunostaining for Wnt5a, Ror2, CTHRC1, and E-cadherin. Statistical significance was tested at an alpha of 0.05. The software PASW Statistics 18 was used for data analysis (Pearson Education, New York City, NY).

Article Snippet: To one of each of the two sections the following antihuman primary antibodies were applied: mouse monoclonal antibody against Wnt5a (Abcam, Cambridge, MA) applied at 8.33 μg/ml; rabbit polyclonal antibody against Ror2 (Abnova, Taipei City, Taiwan) applied at 1/100 dilution, and mouse monoclonal antibody against CTHRC1 (Abnova, Taipei City, Taiwan) applied at 4.0 μg/ml.

Techniques: Immunohistochemical staining, Expressing, Immunostaining, Software

Journal: Oncotarget

Article Title: Wnt5a / planar cell polarity signaling pathway in urothelial carcinoma, a potential prognostic biomarker

doi: 10.18632/oncotarget.15877

Figure Lengend Snippet: Wnt5a (A) , Ror2 and CTHRC1 (B) , E-cadherin and vimentin (C) , in RT4, J82 and T24 urothelial carcinoma cell lines.

Techniques:

Journal: Oncotarget

Article Title: Wnt5a / planar cell polarity signaling pathway in urothelial carcinoma, a potential prognostic biomarker

doi: 10.18632/oncotarget.15877

Figure Lengend Snippet: Top row: H&E stain shows morphological differences between each cell line. Middle row: confocal microscopy images show the expression of Wnt5a (green) for each cell line. Bottom row: confocal microscopy image of the merge expression of Ror2 (green) and CTHRC1 (red) for each cell line. Although the co-expression and colocalization of Ror2 and CTHRC1 is present in all cell lines, it is clearest for RT4 and J82 cell lines.

Techniques: Staining, Confocal Microscopy, Expressing

Journal: Oncotarget

Article Title: Wnt5a / planar cell polarity signaling pathway in urothelial carcinoma, a potential prognostic biomarker

doi: 10.18632/oncotarget.15877

Figure Lengend Snippet: Primer sequences used for real-time RT-PCR analyses

Techniques: Quantitative RT-PCR

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1) Product Images from "Wnt5a / planar cell polarity signaling pathway in urothelial carcinoma, a potential prognostic biomarker"

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